Craig Venter is going to make synthetic life. Prepare yourself.

Craig Venter is pushing the boundaries of what humans can do with DNA and aims to create new life to serve our needs. At TEDMED this past year, he discussed what he and his company, Synthetic Genomics, have accomplished by analyzing and manipulating the genes of simple organisms. While the presentation was titled “What could we do with synthetic life?” it would have been more apt to call it “What we have done so far is going to blow your freakin’ mind.” Venter gives an in depth look at the methods which will lead to custom made organisms. Biofuel, new antibiotics, vaccines – Venter could one day make them all by using yeast and bacteria to reproduce artificially constructed genomes. As many have said, synthetic biology is likely to be the defining technology of the 21st century, and Venter plans on helping to write that definition. Check out his TEDMED video, in its entirety, after the break.

Long time readers will know that I can never send you into a 18 minute video without a field guide. Here are some highlights so you can skip around as you see fit:

0:23 – Venter opens his talk by discussing how DNA is an analog software device, and details his gene mapping efforts to date.
2:45 – Unlike electronics, synthetic biology has more than 3 or 4 design components. There are 20+ million genes to use as basic parts.
3:15 – The simplest bacterium (genetically) has just 580 kb of data – about 500 genes. Venter wanted to find the most basic form of life you can make.
4:25 – If DNA = software, how do you boot it up? It took Venter more than a decade to artificially assemble the genome of a simple virus (5000 genes).
5:35 – But injecting bacteria with genes allows that DNA to be replicated very quickly. Software builds hardware in biology. At 100k genes, however, bacteria are no longer great at assembling DNA pieces.
7:22 – Yeast can repair and assemble long pieces of DNA with overlapping ends. All you really need is an artificial centromere which Venter’s team happily created. Mass production of new life using yeast is possible.
9:30 – By injecting DNA, you can change one species into another. Incredible!
11:30 – To form bacteria out of DNA assembled in yeast, you have to methylate it.
13:45 – We now have the pieces for making synthetic life. You assemble DNA in yeast, change/build it as you see fit, then transplant into bacteria which will change species.
14:40 – Venter says it is still possible that Synthetic Genomics will create a new species in 2010, but he warns that he said the same thing for the past two years.
15:00 – computer software that designs biological software
15:46 – The huge range of design components could allow for amazing medical advancements. Antibiotics based on ocean organisms, new vaccines, etc. A meningitis B vaccine is in phase III trials in Europe.
17:26 – Venter ends by mentioning the ongoing ethical discussions concerning synthetic life.

After watching Venter speak, I don’t wonder if synthetic life will become a reality, I wonder when. There are few technologies as awesome as those that create new organisms. The inherent self assembly capabilities of biology make synthetic life a revolutionary and possibly democratizing innovation which we are likely see bear fruit in the next decade. What ever does or does not happen, you can bet Venter will be at the heart of it.

[screen capture and video credit: TEDMED]

Now for the bad news. You’ve got competition. Some of the best scientists across the globe are chasing the holy grail of biology, and they’re making some serious headway. The prospect of man-made life is becoming less a question of if, and more a question of when. But can gene engineering really save the world? Can it destroy it?

Welcome to the wonderful world of synthetic genomics.

The Idea

The first step to cooking up your own life form is to understand the language it’s written in: DNA. The genetic revolution of the past few decades has allowed scientists to sequence whole genomes, from fruit flies and rats to our very own species. Once the genomes are mapped, the task becomes making a synthetic copy, A by T by C by G. DNA gets stitched together using a combination of different lab techniques, with the final goal of building a whole genome. Different research teams have taken different approaches, and the race is on to see who succeeds first.

Craig Venter with a sweet scarf

Craig Venter is something of an overachiever. Not content with his critical role in the Human Genome Project, he’s now running the J. Craig Venter Institute (JCVI) and the startup Synthetic Genomics. Both groups are at the forefront of a whole host of genetic engineering projects, not least of which is the race towards synthetic life. Their starting point is mycoplasma genitalium, a parasitic bacterium that boasts one of nature’s smallest genomes: perfect for hacking.

In January 2008, JCVI announced that they had successfully synthesized the 580,000 base-pair genome of the bacterium. This was a huge milestone towards man-made life, but they haven’t crossed the finish line yet. The final step is to boot up the software: insert the genome into a cell and bring it to life. This unrealized goal already has the name mycoplasma laboratorium, and would replicate normally within the environment of a surrogate cell.

George Church, a molecular geneticist at Harvard Medical School, is taking a different approach. His focus is E. coli, which has a simpler genome that can arguably be easier (and faster) to synthesize. Nothing like some healthy competition between a few of the world’s best geneticists.

Building Something New

Venter and co. are working to hone down the bacterium’s genome to its bare bones, the smallest amount of DNA that can sustain life (called the Minimal Genome Project). Once they have the skeleton of a life form, they can hypothetically fill it in with genes to customize its behavior. This opens the door for turning bacteria into little factories for useful products: biofuels, medicine, you name it.

The more Legos in your box, the cooler stuff you can build. Making a genome is no different: the more genes at your disposal, the more design options will be available. Currently, about 20 million different genes have been discovered throughout the natural world. Venter and others are scouring the planet for life forms with unique properties – say, surviving in a nuclear reactor – whose genes can be added to the database.

Synthetic Genomics is also working on genetically modifying algae to serve as a source of biofuel, which they hope will replace the petroleum industry as mankind’s primary energy source within a decade. Feeding on just sunlight and carbon dioxide, the algae could be grown in your very own backyard. Using CO2 instead of sugar as a source of carbon would not only solve the food-for-fuel problem, it could help reduce greenhouse gases currently cooking the planet. A few other startups, George Church’s LS9 and Amyris Biotech, are racing to develop biofuels before Synthetic Genomics; LS9 looks to be leading the pack. Curiously, BP has teamed up with Venter to produce the biofuels he hopes will make the petrol giant obsolete. That is, if Dick Cheney doesn’t kill him with a shotgun first.

So how far along is all of this? It’s hard to say. Venter occasionally makes cryptic remarks about the JVCI pipeline, leaving the rest of us to speculate. In February 08, he claimed an algae-based fuel product could be ready by August of this year (no word as of yet). Last year Venter also gave the vague promise that Synthetic Genomics would have “multiple things on the market within five years.” The synthetic life project is going to take longer. Dr. David Deamer at UCSC recently claimed it could be done within five to ten years, and most estimates fall within that relatively wide span of time.

Still, progress is being made all the time. George Church created an artificial ribosome earlier this year, a key component to expressing genes into proteins. A newly developed metallofullerine could provide the template for developing amino acids and RNA. With so many scientists chasing the same goal across the planet, more eureka moments are sure to come. The only question remaining is how long it will take for these advances to arrive. Realistically, it could be many years before synthetic genomics can reach its potential. But if there’s a field known for surprises, genetics takes the cake.

The End Is Nigh

There’s no shortage of chatter about the possible implications of synthetic life. Proponents hail it as a second genesis, an outstanding scientific achievement that could solve the ever-looming dangers of global warming, peak oil, and pandemic disease. To be sure, genetic engineering has a wide scope of applications. Venter, in characteristically dramatic form, claims its limits are those of our imagination.

But keeping such powerful technology in well-meaning hands is no guarantee.  Tinkering with the code of life could lead to some devastating weapons: bacteria and viruses custom built to kill. Regulating the frontier of biotechnology is nearly impossible, and critics warn that synthetic life could revolutionize DIY bioterrorism.

As with any revolutionary technology, synthetic life is both frightening and hopeful in the potential it suggests. That might sound cliché, but it’s cliché for a reason: the world is changing rapidly, and the dizzying pace of innovation should both excite us and give us pause. The ways in which we deploy our new tools will shape the outcome of these times, as well as those to come. Rest assured: life from a laboratory will rock the world in one way or another.

So stay tuned.